FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004890232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004890232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17769071
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG26550.034.33267245
TATGCCG28350.032.361248
CTCGTAT30550.030.22363944
CGTATGC30400.030.0819146
AGTCACG39400.023.28521528
GTATGCC39900.023.21528647
GATCGGA40300.023.0841081
CGTCCGC41950.022.2910233
ACGTCCG41650.022.2390432
GTCCGCA42250.021.923934
ACGTCTG43100.021.69618615
CGCACAT42950.021.56658437
TCCGCAC44400.021.06158835
ACACGTC45400.020.27216713
CGTCTGA47350.019.31270616
CACGTCT50750.018.2513514
ATGCCGT51000.018.10475549
CCGCACA55300.017.17693136
CACACGT56200.016.74386812
TCGGAAG59650.016.0233423
Produced by FastQC (version 0.11.5)