FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7208178
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA24450.098.9313051
ACGATAC18500.092.94261
CGTTAGA30000.091.071921
ATTACGA15250.090.1660849
ACAACGA18450.086.233417
ATGCGAT16250.085.187281
TACACGA24100.084.950081
CTCTACG31950.081.864971
GATCTAG28650.081.3766561
CGCTATC32000.081.054011
TCGGTAA26600.080.800651
CAACTAG30150.079.986211
ATAGACC30750.079.847121
ATCCGAA34300.079.654751
GATCGTA19500.078.088341
CGGTTAA23900.077.430191
TACGGGC37400.076.558851
CATTACG18050.076.17918
ACATACG18750.075.771841
ATAAGTC27500.075.650483
Produced by FastQC (version 0.11.5)