FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7134917
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA23600.094.040521
ATTACGA15200.092.3642049
ATGCGAT15050.091.380281
CGTTAGA32000.088.910681
CTCTACG30400.085.451911
ATAGACC32150.084.874541
CAACTAG30500.084.456021
ACGATAC20950.084.401361
GATCTAG29600.083.582311
CCGTACT35150.083.427051
ATCCGAA32900.083.160891
ACAACGA22200.082.698937
TACACGA25200.082.294631
TCGGTAA26500.081.003411
TACGGGC39450.080.051581
GATCGTA19000.080.042351
CAACGAT33100.078.957128
ACGGGCT65000.078.922881
CGTCCAT32950.077.513771
CGATAGG13750.076.9672554
Produced by FastQC (version 0.11.5)