FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7134917
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1750492.453413263251696No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT365290.5119751217848785No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT146950.20595894808587123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT271700.0113.537931
AGCAGTG296750.0103.8491442
GTATCAA330950.092.951049
GGTATCA335250.091.933858
CAGTGGT336850.091.0295264
TGGTATC337500.090.788937
GTGGTAT343300.089.239446
AGTGGTA365800.083.923715
GCAGTGG383700.080.608453
CTACACG6450.026.0914461
AGAGTAC330050.025.39068615-19
AACGCAG345000.024.95618610-14
CAACGCA336900.024.82968710-14
TCAACGC330800.024.48235110-14
GTACTTT331450.023.28409420-24
CAGAGTA356100.022.85811415-19
GCAGAGT363600.021.812515-19
ATCAACG327500.021.1909079
TACTTTT390650.020.57748820-24
TATCAAC371200.018.6774398
Produced by FastQC (version 0.11.5)