FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7916220
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1935282.4447021431946054No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT401500.5071865107336583No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT162130.20480734491967126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT291800.0112.5810241
AGCAGTG321400.0102.1984942
GTATCAA353250.092.784739
GGTATCA359300.091.206398
TGGTATC362100.090.121097
CAGTGGT365700.089.685564
GTGGTAT369750.088.529096
AGTGGTA393050.083.628565
GCAGTGG414500.079.439293
CTACACG8050.029.0777991
AGAGTAC356650.025.19901315-19
CAACGCA360350.024.80904810-14
AACGCAG374250.024.65322110-14
TCAACGC353050.024.36362610-14
GTACTTT356650.023.15049420-24
CAGAGTA384500.022.84207215-19
GCAGAGT395900.021.44606415-19
ATCAACG349800.021.4029949
TACTTTT427150.020.19929320-24
TACACGA10150.019.8621882
Produced by FastQC (version 0.11.5)