FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7925163
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1990862.5120745150604473No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT416380.5253898247897235No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT165290.20856353364593258No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79310.10007365148199476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT297950.0112.4527741
AGCAGTG328200.0102.551622
GTATCAA360600.093.319729
GGTATCA366750.091.695968
TGGTATC370650.090.3426367
CAGTGGT374050.089.80794
GTGGTAT377300.088.845186
AGTGGTA402450.083.6134645
GCAGTGG423150.079.999593
CTACACG8100.028.8317661
AGAGTAC363300.025.5099915-19
CAACGCA368250.024.87551910-14
AACGCAG382100.024.82171810-14
TCAACGC360550.024.61604910-14
GTACTTT365750.023.36820420-24
TACACGA10500.022.6268482
CAGAGTA397450.022.58635315-19
GCAGAGT399800.021.80897515-19
ACGACGC11300.021.662075
ATCAACG357550.021.2837739
Produced by FastQC (version 0.11.5)