FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8030328
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA28950.096.89651
ATGCGAT17450.089.771611
ACGATAC23150.089.069991
CGTTAGA36450.087.75321
CTCTACG32600.084.259671
ACAACGA24100.083.925977
TCGGTAA30550.083.23581
ATTACGA16000.081.426499
ATAGACC32400.080.507061
ATCCGAA38950.080.063071
CGCTATC34400.079.850721
CAACTAG29700.078.2582551
TACGGGC40750.077.599341
CGTCCAT33950.077.475321
GATCTAG34650.077.38211
GCATGCG31600.077.242031
AACGTTG30650.076.545791
ACGGGCT72400.076.081531
TACACGA27700.075.754541
CGAACAT26000.075.3832551
Produced by FastQC (version 0.11.5)