FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8022316
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA30200.093.734111
ATGCGAT19100.088.010331
ACGATAC22200.085.226641
CGTTAGA35100.085.209831
CGATAGG15800.084.747024
ACAACGA25950.083.783237
CTCTACG33100.083.762951
GATCGTA21550.083.069731
CAACTAG31000.082.629661
ATAGACC32450.082.525481
GATCTAG32200.082.036241
TCGGATG31150.080.197158
TACACGA30050.080.1564561
ATCCGAA39900.079.518581
ATTACGA15650.079.1227959
CAACGAT39250.078.870818
CGCTATC34900.078.400241
TCGGTAA31750.078.15661
CCGTACT35750.077.35041
TACGGGC44100.077.060751
Produced by FastQC (version 0.11.5)