FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6653169
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1648292.477450971108655No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT340090.5111699402194654No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT136660.20540587500482854No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67210.10101952918977408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT247400.0111.339051
AGCAGTG268650.0102.535342
GTATCAA296150.093.021619
GGTATCA299400.092.038668
TGGTATC303600.090.505947
CAGTGGT306200.089.642724
GTGGTAT310150.088.826676
AGTGGTA331700.083.141935
GCAGTGG350100.078.854543
AGAGTAC296650.025.48066515-19
CAACGCA302500.024.82569910-14
AACGCAG314000.024.63177310-14
TCAACGC295700.024.48123210-14
GTACTTT298300.023.26718320-24
CAGAGTA320200.022.97261215-19
CTACACG6350.021.8908211
ATCAACG292850.021.7292219
GCAGAGT326350.021.72352215-19
TACTTTT353100.020.45956420-24
TATCAAC331650.019.27458
Produced by FastQC (version 0.11.5)