FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6761810
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGA14700.094.010959
ATGCGAT15000.093.275671
CGATTGA22650.092.979681
ACGATAC19700.090.257241
CGTTAGA26850.089.83431
TACACGA23650.087.199451
GATCGTA18850.085.43571
CAACTAG24750.082.1461941
ATCCGAA35050.080.876071
TACGGGC35450.080.785751
CTCTACG26750.080.36311
ACAACGA19350.080.3465657
TCGGTAA27350.080.1987461
CGCTATC31700.079.0783541
ACGGGCT59800.078.598991
ATAGACC29450.077.944181
CCGTACT33600.077.209221
CTGATAG28150.077.142961
CGGTTAA25450.077.023441
TCGGATG25150.076.41318
Produced by FastQC (version 0.11.5)