FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6761810
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1692002.5022885884104995No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT351050.5191657263365874No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT140100.20719304446590484No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68320.10103803567388021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT257250.0112.728991
AGCAGTG283550.0102.620392
GTATCAA310650.093.172559
GGTATCA314350.092.098778
TGGTATC319950.090.013017
CAGTGGT322400.089.624524
GTGGTAT325500.088.417776
AGTGGTA345950.083.524095
GCAGTGG367400.079.2740863
CTACACG6650.027.4059311
AGAGTAC313950.025.29990815-19
AACGCAG329550.024.72252810-14
CAACGCA319850.024.7115810-14
TCAACGC312350.024.43839810-14
GTACTTT313950.023.40420520-24
CAGAGTA339600.022.78276415-19
GCAGAGT346600.021.72033515-19
ATCAACG309050.021.0088869
TACTTTT376350.020.28522520-24
ACGACGC10150.019.1489725
Produced by FastQC (version 0.11.5)