FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11371528
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2758432.4257338151917667No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT573020.5039076542747817No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT227310.1998939808264993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT418050.0112.927391
AGCAGTG464450.0101.854682
GTATCAA513950.091.8045659
GGTATCA520000.090.737248
TGGTATC526400.089.374247
CAGTGGT535300.087.8835144
GTGGTAT539750.087.311926
AGTGGTA574100.082.394895
GCAGTGG606200.078.1658863
CTACACG12000.035.2889371
TACACGA15700.027.0628172
ACGACGC16050.026.4626465
AGAGTAC513850.025.38356415-19
CAACGCA527850.024.80469110-14
AACGCAG546850.024.63003210-14
TCAACGC514350.024.57399210-14
GTACTTT517950.023.4063220-24
CAGAGTA560250.022.80065515-19
ATCAACG509550.022.6443129
GCAGAGT570250.021.80235515-19
Produced by FastQC (version 0.11.5)