FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7980539
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGA17400.088.954538
CGATTGA22300.088.242251
TTACGCA19500.087.8616643
TCGGTAA32450.087.817231
CCGTACT38800.087.7208561
ATGCGAT22150.087.523681
CGTTACG21000.085.703699
CGAACAT42500.085.057071
TACGGAT23050.085.054831
ATCCGAA44500.084.673631
GACGTTA35950.084.538561
CTAATGG35850.084.1644741
GTAACGT46500.083.696251
CGTCGAA14600.083.332187
TAAACCG24400.083.0371861
TCGTTAC29550.082.5887768
ACGATAC21550.081.84381
GATCGCG37450.080.179441
CTTACGC22100.079.854172
CAACTAG28950.078.545931
Produced by FastQC (version 0.11.5)