FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9828471
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT26950.093.918771
TTACGCA24950.090.3108143
TACGGAT30800.088.082581
CCGTACT46900.086.380211
CGAACAT47050.085.795641
GATCGTA34750.084.977341
CGTTACG28150.083.628259
ACGATAC25300.083.369921
TCGTTAC36800.083.338458
CAATCGA19400.083.124678
CTTACGC27850.082.988652
GACGTTA42800.082.929291
CGATTGA28300.082.756351
GATCGCG42300.082.7059251
ATCCGAA51400.082.355341
CGTCGAA18550.081.885457
CTAATGG39750.080.3265841
ACGTCAA45500.080.246151
GAACCTA52450.079.4586261
GTAGCGT42350.079.04668
Produced by FastQC (version 0.11.5)