FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8303356
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2746193.307325375426514No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT477510.5750807263954478No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT218260.2628575722876389No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT101530.12227586050748637No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88460.10653523707763464No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85150.10254889709654746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT398800.0115.9134451
AGCAGTG432300.0107.3738562
GTATCAA478600.097.051159
GGTATCA484450.095.803768
CAGTGGT488850.094.4510654
AGTGGTA490500.094.2947855
TGGTATC490050.094.2236947
GTGGTAT494150.093.540016
GCAGTGG570650.081.391323
AGAGTAC478200.026.01490815-19
AACGCAG497200.025.58904810-14
CAACGCA486150.025.4214210-14
TCAACGC476450.025.13215810-14
GTACTTT488300.023.71923620-24
CAGAGTA528950.022.95550315-19
GCAGAGT528650.022.40752615-19
TACTTTT555850.021.569920-24
ATCAACG471500.021.48446510-14
CGCAGAG517950.019.54326415-19
CTACACG7900.019.3786471
Produced by FastQC (version 0.11.5)