FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8311878
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2722253.2751322865903467No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT469370.5646978937852553No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT214180.257679431772218No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT98810.11887806822958663No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87490.10525900404216712No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84780.10199860970047925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT403700.0116.7980651
AGCAGTG437500.0107.790652
GTATCAA476850.098.8552559
GGTATCA486200.097.035248
AGTGGTA486350.096.770795
TGGTATC488100.096.18847
CAGTGGT492350.095.6224754
GTGGTAT491300.095.612736
GCAGTGG570400.082.8536453
AGAGTAC473200.026.03896515-19
AACGCAG494850.025.47836710-14
CAACGCA483600.025.35654810-14
TCAACGC472450.025.2144510-14
GTACTTT483300.023.73803920-24
CAGAGTA528600.022.74619315-19
GCAGAGT518750.022.72574615-19
ATCAACG467350.021.85747510-14
TACTTTT549250.021.56145920-24
CGCAGAG510300.019.90856615-19
ACGCAGA483300.019.35567515-19
Produced by FastQC (version 0.11.5)