FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7620472
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT20800.093.6493151
ATCCGAA41200.089.2660751
TTACGCA17750.089.2271963
CGATTGA23100.089.04461
CGAACAT36650.087.854281
CCGTACT36500.085.825741
TCGGTAA33400.085.739881
CGTTACG19900.085.37699
CTTACGC19300.083.564272
GATCGTA27400.083.293721
ACGATAC20250.082.912511
TCGTTAC26600.082.00548
ACGTCAA36000.081.7684251
CGCTATC31600.081.1934051
CTAATGG32550.080.386781
TACCTTA37550.080.32881
TACGGAT24450.080.263491
GATCGCG33850.079.87621
CGTCGAA13850.079.526997
GTAACGT42250.078.7901461
Produced by FastQC (version 0.11.5)