FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7620472
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2514353.2994675395434827No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT430850.5653849262880304No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT200850.26356635127062994No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT90770.11911335675795409No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79880.10482290335821719No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77300.10143728629932634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT372150.0117.184491
AGCAGTG403150.0108.1996152
GTATCAA444300.098.556489
GGTATCA451650.096.78438
AGTGGTA454250.095.949485
TGGTATC457100.095.299617
GTGGTAT458700.095.112186
CAGTGGT457050.095.074394
GCAGTGG522850.083.618863
AGAGTAC441900.026.13491615-19
AACGCAG459800.025.6684210-14
CAACGCA450250.025.51244410-14
TCAACGC441600.025.28499810-14
GTACTTT449900.024.00656520-24
CAGAGTA488050.023.1001115-19
GCAGAGT487150.022.61667815-19
TACTTTT507050.021.93118520-24
ATCAACG437550.021.7474610-14
CGCAGAG480500.019.79528415-19
ACGCAGA453300.019.34090615-19
Produced by FastQC (version 0.11.5)