FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7642841
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT19950.098.6293951
CGATTGA22300.089.542911
CGTTACG22850.088.850449
TCGTTAC31300.088.7858
CCGTACT36700.087.371981
CTAATGG32300.084.8341751
ACGTCAA35300.084.2308041
TAAACCG24250.082.943591
TCGGTAA33150.082.878781
CGTCGAA13500.082.659847
ATCCGAA39700.082.238091
ACGATAC20000.081.985691
CGAACAT42150.081.953271
TACGGAT23450.081.733181
GACGTTA34000.081.021151
GATCGTA28700.080.493881
TTACGCA17850.080.2538763
TACGGTA25300.079.213221
CGCTATC34650.078.870311
CGATCGG41400.078.333071
Produced by FastQC (version 0.11.5)