FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7642841
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2539723.323005149524895No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT438680.5739750440968221No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT199350.26083232661781136No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT93310.12208810833563069No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81410.10651798199125169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT368400.0117.211911
AGCAGTG398700.0108.094372
GTATCAA440800.098.146759
CAGTGGT445800.096.440194
GGTATCA449950.096.103518
AGTGGTA451750.095.7855765
TGGTATC451450.095.4959267
GTGGTAT454950.094.999856
GCAGTGG521050.082.8588643
AGAGTAC436300.025.9408215-19
AACGCAG455350.025.52745810-14
CAACGCA446550.025.27278510-14
TCAACGC436200.025.05382710-14
GTACTTT445300.023.65157120-24
CAGAGTA479500.023.03013615-19
GCAGAGT483050.022.26767515-19
ATCAACG431350.021.77386510-14
TACTTTT504500.021.64971220-24
CGCAGAG474750.019.72400715-19
ACGCAGA446950.019.33674815-19
Produced by FastQC (version 0.11.5)