FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7594151
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT21350.089.9566041
CGATTGA21100.089.643321
CAATCGA16550.087.873048
ATCCGAA41750.087.821541
TCGTTAC27150.087.507728
CGAACAT39850.086.7146151
ACGATAC20050.084.541241
GACGTTA33700.084.4061051
TCGGTAA33550.084.132971
GATCGTA27500.083.3306661
CCGTACT38600.083.303261
CGTTACG20750.082.230429
TACGGAT21950.081.8633351
CGTCGAA13400.081.6659557
AGCGATA17550.081.635348
GCATGCG35300.080.786421
TAAACCG23650.080.592971
GTAGCGT29750.080.343998
CTAATGG31850.079.7155461
ACGTCAA34650.078.312781
Produced by FastQC (version 0.11.5)