FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10025640
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2492852.486474678923241No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT492420.491160664057357No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT201250.2007353146532291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT393900.0112.320071
AGCAGTG436450.0101.47382
GTATCAA464750.095.400619
GGTATCA475650.093.30948
TGGTATC475450.092.501187
CAGTGGT482900.091.2213754
GTGGTAT483300.091.2072756
AGTGGTA493750.089.494295
GCAGTGG584200.075.945843
AGAGTAC459650.025.67385315-19
CAACGCA473400.024.97671510-14
AACGCAG489600.024.92666410-14
TCAACGC462900.024.75009310-14
GTACTTT466650.023.3203120-24
CAGAGTA509250.022.52862415-19
GCAGAGT511800.021.77775815-19
ATCAACG459350.020.91037610-14
TACTTTT549950.020.55000520-24
CTACACG10300.019.8730831
CGCAGAG505150.018.88917415-19
Produced by FastQC (version 0.11.5)