FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10175999
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT27100.0100.3371661
TAAACCG37600.097.521951
CGATTGA31550.096.813721
TCGTTAC31100.094.89568
TACGGTA46900.094.8154751
GTAACGT43500.088.149281
CGAACAT38000.088.054451
GTTAAGC49750.086.8928151
TTTATGC63150.083.115381
CTAATGG36700.081.83771
GATCGTA22750.081.711091
TCGTAGA35750.081.5654451
GTCCGAA43700.081.370718
TACCTTA52150.081.216461
TCGGTAA42800.080.904391
TACGGAT30850.080.5789261
CTCTACG49600.079.365971
CGATCGG48350.079.005451
CGAATGT42550.078.981181
CTGATAG46150.078.190821
Produced by FastQC (version 0.11.5)