FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10220857
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2545932.49091636836324No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT499000.48821737746648836No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT206220.2017639029682149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT395550.0112.443841
AGCAGTG437700.0101.866892
GTATCAA474500.094.018999
GGTATCA484700.091.996358
TGGTATC486600.091.460057
GTGGTAT494550.090.0219966
CAGTGGT498150.089.181824
AGTGGTA503550.088.456355
GCAGTGG591000.075.9138263
AGAGTAC473150.025.46187215-19
AACGCAG494600.025.08636510-14
CAACGCA482200.024.89824310-14
TCAACGC472450.024.63175610-14
GTACTTT482250.023.1141220-24
CAGAGTA520750.022.5234315-19
GCAGAGT528100.021.5828615-19
ATCAACG466550.020.79787310-14
TACTTTT559900.020.5437720-24
TATCAAC509500.019.03258
CGCAGAG518400.018.67592615-19
Produced by FastQC (version 0.11.5)