FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10249158
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT28650.099.253431
TAAACCG35700.097.830181
CGATTGA33300.094.5899661
TACGGTA48150.093.280311
TCGTTAC32600.092.531558
GTAACGT44850.088.6012041
CGAACAT39550.086.6476751
GTTAAGC45900.085.938971
TTTATGC63700.085.847451
TACCTTA52200.085.0652851
TACGGAT29100.084.1883241
CCGTACT40150.082.9921
CTCTACG50650.082.198181
CGAATGT45800.081.509841
CTAATGG39150.080.455841
GATCGTA23600.080.327991
TGACTAG47100.080.188931
CGCGATA15650.080.073212
CGATCGG47350.079.765551
TCGTAGG25200.079.4214258
Produced by FastQC (version 0.11.5)