FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8935235
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2168282.4266625332182086No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT423280.47372005324985855No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT175130.19599932178616455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT344100.0111.2977451
AGCAGTG385400.099.684692
GTATCAA409100.094.3210459
GGTATCA416550.092.518838
TGGTATC420100.090.9461447
GTGGTAT425300.089.969596
CAGTGGT429350.089.13274
AGTGGTA434750.088.243825
GCAGTGG510400.075.65573
CTACACG10350.030.3820151
AGAGTAC406300.025.3770615-19
CAACGCA417200.024.89434210-14
AACGCAG430700.024.81276110-14
TCAACGC408300.024.59766410-14
TACACGA12550.023.521472
GTACTTT411250.023.11737420-24
CAGAGTA447900.022.49610715-19
ACGACGC13800.021.9103015
GCAGAGT455250.021.53514915-19
ATCAACG401600.020.93145610-14
Produced by FastQC (version 0.11.5)