FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9137800
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT28100.0101.1512151
CGATTGA28950.096.67471
TCGTTAC30450.095.989698
TAAACCG35050.095.612261
TACGGTA41900.092.6462551
CGAACAT35450.090.022061
GTAACGT39300.088.4170461
TACGGAT28300.087.07911
TTTATGC55550.086.8929751
GTTAAGC42200.086.1307451
CTCTACG48950.084.2036741
TCGTAGG22300.082.9686668
CTAATGG36100.082.964191
CGATCGG41900.080.848611
CTGATAG40600.080.751611
CGTTAGA34000.080.60521
TCGTAGA32400.079.874031
CAACTAG31950.079.406341
CGCGATA13200.079.088482
TACCTTA47300.077.919741
Produced by FastQC (version 0.11.5)