FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9412649
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT29150.099.068411
TAAACCG37850.098.454051
TACGGTA42700.094.444321
CGATTGA29000.091.5339051
TTTATGC55800.089.1311
TCGTTAC28300.087.5089658
CGAACAT36900.086.364181
GTAACGT40900.085.584781
CTAATGG35750.084.654611
CGAATGT38800.083.826581
GTTAAGC43800.083.747611
GATCGTA21350.083.684841
TCGGTAA40650.083.5995561
CGTTAGA32800.083.375031
CTGATAG41650.081.0670851
TCGTAGA33850.080.78881
GATCGCG46300.080.6431661
TACCTTA47300.080.1716461
CTCTACG47750.079.8742751
TACGGAT29250.079.0336461
Produced by FastQC (version 0.11.5)