FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9534233
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG33200.099.02081
ATGCGAT26850.098.820041
CGATTGA30850.097.5849461
TCGTTAC30200.092.470388
CGAACAT37200.091.508591
TTTATGC58850.091.163141
TACGGTA42000.089.554691
GTTAAGC43900.088.50151
CTCTACG46650.087.1907651
GTAACGT40400.085.3429641
TACGGAT28450.083.782521
TCGGTAA37900.082.7022251
CTGATAG40650.082.666271
CTAATGG34200.082.484581
TACCTTA50500.080.687891
CCGTACT39300.080.683491
CGCGATA14300.080.050542
ACCGAAT29350.079.9442759
CGATCGG46450.079.562821
CGAATGT41250.079.3433151
Produced by FastQC (version 0.11.5)