FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9570697
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2345182.45037534883823No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT465340.48621328206294695No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT193640.2023259121044162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT371700.0112.871271
AGCAGTG413450.0101.433872
GTATCAA447950.093.703869
GGTATCA456250.091.975758
TGGTATC457400.091.3362357
GTGGTAT462000.090.476416
CAGTGGT464250.089.8334964
AGTGGTA471700.088.417445
GCAGTGG554750.075.879153
AGAGTAC441900.025.59476715-19
AACGCAG466700.025.0053410-14
CAACGCA456850.024.8573210-14
TCAACGC449500.024.4564610-14
GTACTTT452000.023.20425820-24
CAGAGTA487150.022.66461615-19
GCAGAGT497000.021.58094815-19
ATCAACG443050.020.69117210-14
TACTTTT528800.020.47682420-24
CTACACG9650.020.3545971
TATCAAC481050.018.8864928
Produced by FastQC (version 0.11.5)