FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6609809
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT19200.0104.5135651
TCGTTAC21100.096.218628
CGATTGA20450.093.503371
TAAACCG23600.092.113661
CGAACAT25750.090.916661
TACGGTA28550.090.4038851
CTCTACG33150.089.044541
GTTAAGC31350.087.663551
CTAATGG26650.086.209411
CTGATAG29850.084.518141
TACGGAT21200.084.022391
TTTATGC43900.083.6357041
GTAACGT28000.083.35121
TCGTAGA22300.081.181921
AGCGATA15150.080.784648
TCGTAGG14400.080.4925848
CGATCGG30500.079.618031
GATCGCG32800.079.1332861
CACGAGT22300.079.089873
CGCGATA10200.079.054532
Produced by FastQC (version 0.11.5)