FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6637500
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1701232.5630583804143123No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT338180.5094990583804143No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT137180.20667419962335218No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT67990.1024331450094162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT264900.0112.302891
AGCAGTG292150.0101.961292
GTATCAA312750.095.103069
GGTATCA321000.092.72758
TGGTATC323850.091.622497
CAGTGGT325200.091.051674
GTGGTAT329250.090.274216
AGTGGTA332500.089.565025
GCAGTGG385600.077.40863
CTACACG5450.026.796951
AGAGTAC310300.025.74673815-19
AACGCAG326400.025.16637410-14
CAACGCA319900.024.94407710-14
TCAACGC313350.024.66595610-14
GTACTTT317150.023.24787520-24
CAGAGTA343950.022.62913315-19
GCAGAGT348150.021.70262115-19
ATCAACG309650.020.9524710-14
TACTTTT373050.020.47841520-24
CGCAGAG338400.018.99170915-19
Produced by FastQC (version 0.11.5)