FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13458666
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT44600.0111.00421
ACGATAC42650.0101.0794451
CGATTGA52200.093.7285161
CAACTAG58800.089.8843841
GATCGTA31500.089.315651
TACGGGC73350.088.4080661
AACGTTG48250.086.93721
CGTAACA41300.086.805153
GATCTAG53950.085.163361
ACGAGCC68750.084.9121861
CCGTACT64400.084.89051
GAACCTA72350.084.4049451
AGCGATA31200.084.22268
GACGTTA57350.082.396191
CATCGAA74000.081.5405351
ACGGATC47250.080.901933
AGTACGT48950.080.891048
CGTAGCG77450.079.879491
TACACGA43400.079.064121
TACCTTA64800.078.532641
Produced by FastQC (version 0.11.5)