FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13365605
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT42250.0109.6947251
ACGATAC43800.0100.331161
CGATTGA50000.094.001661
TACGGGC72800.091.8453451
CAACTAG60600.091.726271
AACGTTG52500.089.386811
GATCGTA33000.087.7491
GAACCTA73200.087.566471
ACGAGCC70250.087.2044751
CGTAACA40000.087.109453
CCGTACT61150.085.6661151
CATCGAA76100.083.273441
AGTACGT49350.083.0119868
TCGTACG31100.082.414818
CGTAGCG72100.081.0313951
GATCTAG57200.080.5157241
CGTACGG32050.079.971949
AGCGATA29800.079.728458
GACGTTA55350.079.394851
TACCTTA61900.078.2810441
Produced by FastQC (version 0.11.5)