FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8652402
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT25700.0105.2579351
ACGATAC26200.095.134761
CAACTAG38800.092.959761
CGATTGA31250.092.66351
GATCGTA20350.090.781581
TACGGGC45200.090.177461
GAACCTA47350.089.7986151
AACGTTG33750.086.668391
CGTAACA24900.085.948463
AGTACGT33000.085.7288
ACGAGCC45250.085.379511
GACGTTA35300.084.93961
CCGTACT38100.084.277321
CATCGAA50150.082.8845751
GCGACCA40050.080.1739351
AGCGATA20850.080.098798
CGTAGCG48250.078.8554461
TAGTACG24650.078.847597
GATCTAG33150.078.28551
CTGATAG36050.077.885231
Produced by FastQC (version 0.11.5)