FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8805346
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT27400.0109.038571
ACGATAC27950.0105.850051
CGATTGA33950.095.943291
CGTAACA25600.091.382963
GATCGTA21300.089.6331251
CCGTACT43450.088.885911
CAACTAG38150.088.818871
AACGTTG34850.088.6563641
TACGGGC45950.086.7458341
GATCTAG34200.085.86691
TAGTACG24150.085.2417
GAACCTA44300.084.384071
AGTACGT34300.084.3590248
CATCGAA50200.084.337221
AGCGATA23250.083.277748
ACGAGCC44800.082.7916641
CGTAGCG50850.080.106481
TACCTTA41800.079.494191
ATCCGCG31250.078.312049
GTGATAA37150.077.887348
Produced by FastQC (version 0.11.5)