FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10129962
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT33100.0107.775971
CGATTGA37300.098.37851
ACGATAC32350.098.322581
CAACTAG50100.095.8140641
CGTAACA29850.093.607953
AACGTTG37200.090.798591
TACGGGC53000.089.607881
GAACCTA57150.086.675151
ACGAGCC53250.085.762181
GATCGTA23450.084.3077551
CCGTACT47850.084.158541
ACGGATC31450.083.350083
CATCGAA59200.082.934341
AGCGATA24450.082.738388
GATCTAG42100.082.483281
TCGTACG21700.081.280428
GACGTTA43050.081.002011
TACACGA31000.080.718581
TAGTACG23800.080.4607857
AGTACGT34450.080.245668
Produced by FastQC (version 0.11.5)