FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10292309
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2973802.889341934836974No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT719090.698667325281431No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT270080.26240953317666615No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165510.16080939660867158No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT126270.12268384091460914No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118910.11553287022377584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT443700.0116.81821
AGCAGTG488400.0106.233382
GTATCAA588250.088.029519
CAGTGGT587350.087.995194
AGTGGTA592950.087.3375245
GGTATCA594900.087.045328
TGGTATC598500.086.331387
GTGGTAT603150.085.812376
GCAGTGG732550.071.153013
AGAGTAC581950.025.36362815-19
AACGCAG609150.024.96546710-14
CAACGCA594200.024.61929510-14
TCAACGC586750.024.11211610-14
ATCAACG582400.023.724349
GTACTTT588800.023.0146720-24
CAGAGTA626250.022.8888715-19
TATCAAC605950.022.7872098
GCAGAGT634100.021.8039315-19
TACTTTT668050.021.00652320-24
ACTTTTT790400.018.77135820-24
Produced by FastQC (version 0.11.5)