FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10220574
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT30450.0104.158261
ACGATAC32600.097.958351
CAACTAG44450.093.118291
CGATTGA40250.092.3526841
GAACCTA57100.090.324311
CCGTACT47400.089.7784351
TACGGGC54550.086.678851
ACGAGCC54050.086.673181
AACGTTG39400.086.5906751
CGTAACA31200.086.526183
GATCGTA24000.085.473671
AGTACGT36450.083.7473458
CATCGAA54750.081.977671
ACGGATC34250.081.973763
CTGATAG45600.081.038871
TACACGA31000.080.721881
CGTAGCG56150.080.711021
GATCTAG40350.080.58581
TCGTACG22750.080.064848
AGCGATA25650.079.9945148
Produced by FastQC (version 0.11.5)