FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3153147
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT937992.97477409077344No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT226770.7191862605834742No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT85220.2702696702690994No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50660.16066488495461836No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41350.13113882733662593No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37180.11791394438635434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT144550.0118.848141
AGCAGTG160350.0107.1285252
AGTGGTA190650.089.8010945
GTATCAA192250.089.540569
CAGTGGT191500.089.401084
GGTATCA195000.088.275018
TGGTATC195500.087.719217
GTGGTAT196250.087.383976
GCAGTGG236550.072.740233
CTACACG2950.036.9828031
TACACGA4450.025.8967742
AGAGTAC188650.025.72902715-19
AACGCAG197650.025.24327910-14
CAACGCA193950.024.94530510-14
TCAACGC190800.024.51189810-14
ATCAACG190000.024.2511839
CAGAGTA201050.023.53341115-19
GTACTTT193200.023.21563720-24
TATCAAC198700.023.188628
GCAGAGT206700.022.07512715-19
Produced by FastQC (version 0.11.5)