FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3169869
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT958953.0252038806651003No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT227810.7186732322376729No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86020.2713676811249929No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53120.1675779030616092No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39840.12568342729620688No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37590.11858534217029158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT144900.0117.0092851
AGCAGTG159450.0106.6764762
AGTGGTA191300.089.18025
CAGTGGT190350.088.969864
GTATCAA194350.088.557079
TGGTATC194200.087.92267
GGTATCA195450.087.839088
GTGGTAT195200.087.252296
GCAGTGG235450.072.391143
CTACACG3450.029.6430231
AGAGTAC189750.025.52739115-19
AACGCAG198000.025.4429310-14
CAACGCA195000.025.02957510-14
TCAACGC192600.024.23511710-14
TACACGA4002.910383E-1123.4043522
CAGAGTA202600.023.32552115-19
GTACTTT191050.023.11607220-24
ATCAACG192050.022.8635549
GCAGAGT205450.022.21699515-19
TATCAAC199550.021.8602928
Produced by FastQC (version 0.11.5)