FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9580437
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC39500.0100.0260541
CGAACAT44600.091.203251
CTCTACG47150.090.290511
CGTTAGA39050.089.978131
TACACGA33800.089.9353261
GATCGTA20650.086.841231
TACGGAT29950.085.675531
GTACGTA32950.084.51211
CTAATGG35300.084.461651
ATCCGAA48350.084.280341
TAACGAC21250.083.999519
GAACCTA46700.083.823991
CGATGTA48450.082.451341
GTAACGA21850.082.022298
ATTACGA14850.081.9113859
GTAACGT39450.080.380961
ACGCGAA21450.079.86089
ATGCGAT17600.078.695861
GTAGCGT44350.078.061248
CACGCGA22000.077.864298
Produced by FastQC (version 0.11.5)