FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10056566
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC38900.0100.8277741
CTCTACG48800.095.2457051
CGTTAGA39400.094.524311
CGAACAT46850.094.202611
TACGGAT31200.091.405221
TACACGA35600.086.903731
CACGCGA23950.086.862448
GAACCTA50000.086.2150961
GTACGTA38550.084.8176961
ACGCGAA24550.084.737849
ATCCGAA48350.082.788911
TGCGATA23450.082.573469
CTAATGG33800.082.421951
GATCGTA20800.081.066331
ATGCGAT19400.080.870121
CGATGTA46200.079.976891
CGATTGA24850.079.950081
GTAGCGT42650.078.482648
CAACGAT51050.077.977468
CGTAACA20750.077.0796363
Produced by FastQC (version 0.11.5)