FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10185996
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC39850.0103.482781
CGAACAT46700.093.296271
CTCTACG51550.091.585411
TACGGAT33750.089.373041
GAACCTA51600.088.5314941
CGATGTA49400.087.902111
CTAATGG36150.087.067371
GATCGTA23350.086.431661
TACACGA37150.086.0965351
GTAGCGT42950.085.468158
CGTTAGA42800.085.4554751
ATGCGAT21600.084.664231
GTACGTA33250.084.362431
CGATTGA24450.081.9471361
GTAACGT41000.079.96661
CAACGAT52550.079.716378
ATCCGAA49450.079.267651
CGTAACA21350.078.8918843
CATATTC52850.078.855321
AACGTTG32650.078.548781
Produced by FastQC (version 0.11.5)