FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10249134
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC39050.097.935851
CTCTACG50650.094.0240251
CGTTAGA40700.090.749921
TACACGA37100.090.1489941
ATGCGAT19400.089.946961
CGAACAT48900.088.3190841
GTACGTA34750.087.039321
TACGGAT32850.086.9829561
CGATTGA25950.085.455391
GAACCTA50750.084.239981
CGATGTA47950.083.548751
TGCGATA20250.083.188359
GTAGCGT46600.083.112438
CTAATGG37400.082.427341
TAAACCG27750.082.27041
GTCGAAC20450.081.318298
AACGTTG34350.081.0679241
TAGTACG19100.081.035387
CACGCGA25100.080.8807148
GATCGTA21600.080.78571
Produced by FastQC (version 0.11.5)