FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10249134
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1882301.8365454095926543No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT356670.3480001334746916No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT155400.15162256635536234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT322700.0101.133621
AGCAGTG347750.093.943112
GTATCAA349950.093.5117349
GGTATCA361400.090.435778
TGGTATC367200.088.69587
GTGGTAT376650.086.6637046
AGTGGTA381200.085.573475
CAGTGGT384600.084.757914
GCAGTGG484450.067.9571843
AGAGTAC352850.024.91256315-19
TCAACGC350900.024.41481210-14
AACGCAG373650.024.31964110-14
CAACGCA363550.024.2862210-14
GTACTTT360300.022.66372920-24
CAGAGTA407350.021.10581815-19
ATCAACG343950.020.89286610-14
GCAGAGT407250.020.58419815-19
TACTTTT441850.019.15537620-24
CTACACG10550.018.618581
TATCAAC377100.018.3840968
Produced by FastQC (version 0.11.5)