FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10274585
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1923041.871647370672392No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT358490.3489094693362311No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT153950.14983573545792847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT333550.0100.033061
AGCAGTG350050.095.4572452
GTATCAA357950.093.647359
GGTATCA370400.090.539838
TGGTATC379950.087.752197
GTGGTAT385300.086.75796
AGTGGTA390750.085.734565
CAGTGGT392000.085.141714
GCAGTGG479450.070.00643
AGAGTAC363300.024.76861815-19
AACGCAG384400.024.18827410-14
TCAACGC362100.024.1590310-14
CAACGCA375800.024.01005710-14
GTACTTT368250.022.51168420-24
CTACACG10000.021.173191
CAGAGTA414750.021.13707515-19
ATCAACG352300.020.86283710-14
GCAGAGT415650.020.56471815-19
TACTTTT447400.019.35950320-24
ACGCAGA373550.017.9173615-19
Produced by FastQC (version 0.11.5)