FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10318083
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC41250.098.4925161
CGAACAT49400.096.073061
CTCTACG50050.094.099281
GTACGTA35600.089.012681
TACGGAT34450.086.4987951
TACACGA38100.085.2703251
CGTTAGA41600.085.259321
AACGTTG33900.083.8286361
GATCGTA21550.083.641111
GAACCTA52150.083.481051
CGATGTA48150.083.472661
CTAATGG36550.083.119771
CACGCGA23800.082.57978
CGATTGA26350.082.4718861
GTAGCGT49100.081.523148
ACGCGAA24350.080.7144559
ATGCGAT22150.080.062931
ATCCGAA49800.079.977271
TAAACCG28650.079.9100341
CAACGAT50100.078.4589548
Produced by FastQC (version 0.11.5)