FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10349824
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC40200.098.116231
CTCTACG49950.095.020021
TACACGA36300.092.790811
CGAACAT49750.089.833221
CGTTAGA45900.088.632111
CGATGTA45400.086.878241
TACGGAT32900.085.759981
GAACCTA51050.084.8321151
GTAGCGT45400.084.203268
GTACGTA36200.083.58131
TAACGAC22650.082.323119
CAACGAT51950.082.178738
CTAATGG35300.081.168491
CACGCGA25100.080.597748
ATGCGAT18800.080.275341
ACGCGAA25300.079.9605949
GTCGAAC19500.079.7460868
ATCCGAA48900.079.61631
GATCGTA22600.079.0364151
CGATTGA24650.077.7872541
Produced by FastQC (version 0.11.5)