FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004897717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004897717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8686010
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC33900.0102.228711
CTCTACG45400.097.707231
CGAACAT42450.094.0131451
GAACCTA44800.090.38451
CGTTAGA36000.088.361471
GATCGTA18300.085.31821
TACACGA32550.084.9507451
CGATGTA40650.084.89451
TACGGAT28500.083.710881
CACGCGA20600.083.5244758
GTACGTA30350.083.175681
ATGCGAT16800.080.7760541
AACGTTG29100.080.480561
CGATTGA21300.080.152161
TAACGAC17600.079.763649
CTAATGG29450.079.524091
ACGCGAA21700.079.290519
ATCCGAA42950.079.1592561
CAACGAT47450.078.2882468
TGCGATA19300.077.960049
Produced by FastQC (version 0.11.5)